You are here

Biochem Biophys Res Commun DOI:10.1016/j.bbrc.2006.07.122

Imaging of multiple mRNA targets using quantum dot based in situ hybridization and spectral deconvolution in clinical biopsies.

Publication TypeJournal Article
Year of Publication2006
AuthorsTholouli, E, Hoyland, JA, Di Vizio, D, O'connell, F, Macdermott, SA, Twomey, D, Levenson, R, Yin, JALiu, Golub, TR, Loda, M, Byers, R
JournalBiochem Biophys Res Commun
Volume348
Issue2
Pages628-36
Date Published2006 Sep 22
ISSN0006-291X
KeywordsBiopsy, Fixatives, Formaldehyde, Gene Expression Profiling, Humans, In Situ Hybridization, Oligonucleotide Probes, Quantum Dots, RNA, Messenger
Abstract

Gene expression mapping using microarray analysis has identified useful gene signatures for predicting outcome. However, little of this has been translated into clinically effective diagnostic tools as microarrays require high quality fresh-frozen tissue samples. We describe a methodology of multiplexed in situ hybridization (ISH) using a novel combination of quantum dot (QD)-labeled oligonucleotide probes and spectral imaging analysis in routinely processed, formalin-fixed paraffin embedded human biopsies. The conditions for QD-ISH were optimized using a poly d(T) oligonucleotide in decalcified bone marrow samples. Single and multiplex QD-ISH was performed in samples with acute leukemia and follicular lymphoma using oligonucleotide probes for myeloperoxidase, bcl-2, survivin, and XIAP. Spectral imaging was used for post hybridization tissue analysis, enabling separation of spatially colocalized signals. The method allows quantitative characterization of multiple gene expression using non-bleaching fluorochromes. This is expected to facilitate multiplex in situ transcript detection in routinely processed human clinical tissue.

URLhttp://linkinghub.elsevier.com/retrieve/pii/S0006-291X(06)01656-1
DOI10.1016/j.bbrc.2006.07.122
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/16893519?dopt=Abstract

Alternate JournalBiochem. Biophys. Res. Commun.
PubMed ID16893519