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Nature methods DOI:10.1038/nmeth924

Genome-scale loss-of-function screening with a lentiviral RNAi library.

Publication TypeJournal Article
Year of Publication2006
AuthorsRoot, DE, Hacohen, N, Hahn, WC, Lander, ES, Sabatini, DM
JournalNature methods
Volume3
Issue9
Pages715-9
Date Published2006/09/01
ISSN1548-7091
Abstract

The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens.

URLhttp://dx.doi.org/10.1038/nmeth924
DOI10.1038/nmeth924
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/16929317?dopt=Abstract