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Genome-scale loss-of-function screening with a lentiviral RNAi library.
|Publication Type||Journal Article|
|Authors||Root, DE, Hacohen N., Hahn WC, Lander E. S., and Sabatini DM|
|Abstract||The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens.|
|Year of Publication||2006|
|Date Published (YYYY/MM/DD)||2006/09/01|