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Nat Methods DOI:10.1038/nmeth924

Genome-scale loss-of-function screening with a lentiviral RNAi library.

Publication TypeJournal Article
Year of Publication2006
AuthorsRoot, DE, Hacohen, N, Hahn, WC, Lander, ES, Sabatini, DM
JournalNat Methods
Volume3
Issue9
Pages715-9
Date Published2006 Sep
ISSN1548-7091
KeywordsAnimals, Gene Expression Regulation, Gene Library, Genetic Vectors, Humans, Lentivirus, Mice, RNA, Small Interfering
Abstract

The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens.

URLhttp://dx.doi.org/10.1038/nmeth924
DOI10.1038/nmeth924
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/16929317?dopt=Abstract

Alternate JournalNat. Methods
PubMed ID16929317