Prospective isolation and global gene expression analysis of definitive and visceral endoderm.

Dev Biol
Authors
Keywords
Abstract

In spite of the therapeutic importance of endoderm derivatives such as the pancreas, liver, lung, and intestine, there are few molecular markers specific for early endoderm. In order to identify endoderm-specific genes as well as to define transcriptional differences between definitive and visceral endoderm, we performed microarray analysis on E8.25 definitive and visceral endoderm. We have developed an early endoderm gene expression signature, and clarified the transcriptional similarities and differences between definitive and visceral endoderm. Additionally, we have developed methods for flow cytometric isolation of definitive and visceral endoderm. These results shed light on the mechanism of endoderm formation and should facilitate investigation of endoderm formation from embryonic stem cells.

Year of Publication
2007
Journal
Dev Biol
Volume
304
Issue
2
Pages
541-55
Date Published
2007 Apr 15
ISSN
0012-1606
URL
DOI
10.1016/j.ydbio.2007.01.011
PubMed ID
17328885
Links