Extracellular Vesicle Isolation and Analysis by Western Blotting.

Methods Mol Biol
Authors
Emma Kowal
Dmitry Ter-Ovanesyan
Aviv Regev
George Church
Keywords
Animals
Humans
Blotting, Western
Cell Fractionation
Extracellular Vesicles
Abstract

Extracellular vesicles (EVs) are released by mammalian cells and are thought to be important mediators of intercellular communication. There are many methods for isolating EVs from cell culture media, but the most popular methods involve purification based on ultracentrifugation . Here, we provide a detailed protocol for isolating EVs by differential ultracentrifugation and analyzing EV proteins (such as the tetraspanins CD9 , CD63 and CD81 ) by western blotting.
Year of Publication
2017
Journal
Methods Mol Biol
Volume
1660
Pages
143-152
Date Published
2017
ISSN
1940-6029
DOI
10.1007/978-1-4939-7253-1_12
PubMed ID
28828654
Links
Grant list
P50 HG005550 / HG / NHGRI NIH HHS / United States
RM1 HG008525 / HG / NHGRI NIH HHS / United States

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