|Publication Type||Journal Article|
|Year of Publication||2007|
|Authors||Mikkelsen, TS, Wakefield, MJ, Aken, B, Amemiya, CT, Chang, JL, Duke, S, Garber, M, Gentles, AJ, Goodstadt, L, Heger, A, Jurka, J, Kamal, M, Mauceli, E, Searle, SM, Sharpe, T, Baker, ML, Batzer, MA, Benos, PV, Belov, K, Clamp, M, Cook, A, Cuff, J, Das, R, Davidow, L, Deakin, JE, Fazzari, MJ, Glass, JL, Grabherr, M, Greally, JM, Gu, W, Hore, TA, Huttley, GA, Kleber, M, Jirtle, RL, Koina, E, Lee, JT, Mahony, S, Marra, MA, Miller, RD, Nicholls, RD, Oda, M, Papenfuss, AT, Parra, ZE, Pollock, DD, Ray, DA, Schein, JE, Speed, TP, Thompson, K, VandeBerg, JL, Wade, CM, Walker, JA, Waters, PD, Webber, C, Weidman, JR, Xie, X, Zody, MC, Broad Institute Genome Sequencing, P, Broad Institute Whole Genome Assembly, T, Graves, JA, Ponting, CP, Breen, M, Samollow, PB, Lander, ES, Lindblad-Toh, K|
We report a high-quality draft of the genome sequence of the grey, short-tailed opossum (Monodelphis domestica). As the first metatherian ('marsupial') species to be sequenced, the opossum provides a unique perspective on the organization and evolution of mammalian genomes. Distinctive features of the opossum chromosomes provide support for recent theories about genome evolution and function, including a strong influence of biased gene conversion on nucleotide sequence composition, and a relationship between chromosomal characteristics and X chromosome inactivation. Comparison of opossum and eutherian genomes also reveals a sharp difference in evolutionary innovation between protein-coding and non-coding functional elements. True innovation in protein-coding genes seems to be relatively rare, with lineage-specific differences being largely due to diversification and rapid turnover in gene families involved in environmental interactions. In contrast, about 20% of eutherian conserved non-coding elements (CNEs) are recent inventions that postdate the divergence of Eutheria and Metatheria. A substantial proportion of these eutherian-specific CNEs arose from sequence inserted by transposable elements, pointing to transposons as a major creative force in the evolution of mammalian gene regulation.