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Physiol Genomics DOI:10.1152/physiolgenomics.00144.2007

Altered microRNA expression in human heart disease.

Publication TypeJournal Article
Year of Publication2007
AuthorsIkeda, S, Kong, SWon, Lu, J, Bisping, E, Zhang, H, Allen, PD, Golub, TR, Pieske, B, Pu, WT
JournalPhysiol Genomics
Date Published2007 Nov 14
KeywordsAged, Analysis of Variance, Case-Control Studies, Female, Heart Diseases, Humans, Male, MicroRNAs, Middle Aged

MicroRNAs are recently discovered regulators of gene expression and are becoming increasingly recognized as important regulators of heart function. Genome-wide profiling of microRNAs in human heart failure has not been reported previously. We measured expression of 428 microRNAs in 67 human left ventricular samples belonging to control (n = 10), ischemic cardiomyopathy (ICM, n = 19), dilated cardiomyopathy (DCM, n = 25), or aortic stenosis (AS, n = 13) diagnostic groups. miRNA expression between disease and control groups was compared by ANOVA with Dunnett's post hoc test. We controlled for multiple testing by estimating the false discovery rate. Out of 428 microRNAs measured, 87 were confidently detected; 43 were differentially expressed in at least one disease group. In supervised clustering, microRNA expression profiles correctly grouped samples by their clinical diagnosis, indicating that microRNA expression profiles are distinct between diagnostic groups. This was further supported by class prediction approaches, in which the class (control, ICM, DCM, AS) predicted by a microRNA-based classifier matched the clinical diagnosis 69% of the time (P


Alternate JournalPhysiol. Genomics
PubMed ID17712037
Grant ListHL-66582 / HL / NHLBI NIH HHS / United States
P01 HL-074734 / HL / NHLBI NIH HHS / United States