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Genes Dev DOI:10.1101/gad.1613108

A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands.

Publication TypeJournal Article
Year of Publication2008
AuthorsStark, A, Bushati, N, Jan, CH, Kheradpour, P, Hodges, E, Brennecke, J, Bartel, DP, Cohen, SM, Kellis, M
JournalGenes Dev
Date Published2008 Jan 01
KeywordsAnimals, Base Sequence, Body Patterning, DNA, Drosophila, Gene Expression Regulation, Developmental, Genes, Homeobox, Genes, Insect, Genome, Humans, Mice, MicroRNAs, Models, Genetic, Molecular Sequence Data, Nucleic Acid Conformation, RNA, Antisense

MicroRNAs (miRNAs) are approximately 22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression. Here, we report that the miRNA iab-4 locus in the Drosophila Hox cluster is transcribed convergently from both DNA strands, giving rise to two distinct functional miRNAs. Both sense and antisense miRNA products target neighboring Hox genes via highly conserved sites, leading to homeotic transformations when ectopically expressed. We also report sense/antisense miRNAs in mouse and find antisense transcripts close to many miRNAs in both flies and mammals, suggesting that additional sense/antisense pairs exist.


Alternate JournalGenes Dev.
PubMed ID18172160
PubMed Central IDPMC2151017
Grant ListR01 GM067031 / GM / NIGMS NIH HHS / United States
R01 HG004037 / HG / NHGRI NIH HHS / United States
R01 HG004037-01A1 / HG / NHGRI NIH HHS / United States