Scientific Publications

Small-molecule reagents for cellular pull-down experiments.

Publication TypeJournal Article
AuthorsWang, X., Imber BS, and Schreiber SL
AbstractAffinity purification of interacting proteins from cellular extracts is a powerful technique for identifying the cellular targets of small molecules. Affinity matrix-based small-molecule reagents are usually prepared by conjugating small molecules of interest to a solid support such as agarose. This protocol describes an efficient and robust method to immobilize small molecules containing a primary alcohol, a common functional group in small molecules, especially in small molecules prepared using diversity-oriented synthesis. This method comprises one element of a systematic approach to the target identification problem in chemical biology.
Year of Publication2008
JournalBioconjugate chemistry
Volume19
Issue3
Pages585-7
Date Published (YYYY/MM/DD)2008/03/01
ISSN Number1043-1802
DOI10.1021/bc700297j
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/18193824?dopt=Abstract