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Bioconjugate chemistry DOI:10.1021/bc700297j

Small-molecule reagents for cellular pull-down experiments.

Publication TypeJournal Article
Year of Publication2008
AuthorsWang, X, Imber, BS, Schreiber, SL
JournalBioconjugate chemistry
Volume19
Issue3
Pages585-7
Date Published2008/03/01
ISSN1043-1802
Abstract

Affinity purification of interacting proteins from cellular extracts is a powerful technique for identifying the cellular targets of small molecules. Affinity matrix-based small-molecule reagents are usually prepared by conjugating small molecules of interest to a solid support such as agarose. This protocol describes an efficient and robust method to immobilize small molecules containing a primary alcohol, a common functional group in small molecules, especially in small molecules prepared using diversity-oriented synthesis. This method comprises one element of a systematic approach to the target identification problem in chemical biology.

URLhttp://dx.doi.org/10.1021/bc700297j
DOI10.1021/bc700297j
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/18193824?dopt=Abstract