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PLoS Med DOI:10.1371/journal.pmed.0050035

An erythroid differentiation signature predicts response to lenalidomide in myelodysplastic syndrome.

Publication TypeJournal Article
Year of Publication2008
AuthorsEbert, BL, Galili, N, Tamayo, P, Bosco, J, Mak, R, Pretz, J, Tanguturi, S, Ladd-Acosta, C, Stone, R, Golub, TR, Raza, A
JournalPLoS Med
Volume5
Issue2
Pagese35
Date Published2008 Feb
ISSN1549-1676
KeywordsAdult, Aged, Aged, 80 and over, Cell Differentiation, Cells, Cultured, Chromosome Deletion, Chromosomes, Human, Pair 5, Clinical Trials, Phase II as Topic, Erythroid Precursor Cells, Erythropoiesis, Gene Expression Profiling, Genetic Markers, Hematopoietic Stem Cells, Humans, Male, Middle Aged, Myelodysplastic Syndromes, Predictive Value of Tests, Thalidomide
Abstract

BACKGROUND: Lenalidomide is an effective new agent for the treatment of patients with myelodysplastic syndrome (MDS), an acquired hematopoietic disorder characterized by ineffective blood cell production and a predisposition to the development of leukemia. Patients with an interstitial deletion of Chromosome 5q have a high rate of response to lenalidomide, but most MDS patients lack this deletion. Approximately 25% of patients without 5q deletions also benefit from lenalidomide therapy, but response in these patients cannot be predicted by any currently available diagnostic assays. The aim of this study was to develop a method to predict lenalidomide response in order to avoid unnecessary toxicity in patients unlikely to benefit from treatment.

METHODS AND FINDINGS: Using gene expression profiling, we identified a molecular signature that predicts lenalidomide response. The signature was defined in a set of 16 pretreatment bone marrow aspirates from MDS patients without 5q deletions, and validated in an independent set of 26 samples. The response signature consisted of a cohesive set of erythroid-specific genes with decreased expression in responders, suggesting that a defect in erythroid differentiation underlies lenalidomide response. Consistent with this observation, treatment with lenalidomide promoted erythroid differentiation of primary hematopoietic progenitor cells grown in vitro.

CONCLUSIONS: These studies indicate that lenalidomide-responsive patients have a defect in erythroid differentiation, and suggest a strategy for a clinical test to predict patients most likely to respond to the drug. The experiments further suggest that the efficacy of lenalidomide, whose mechanism of action in MDS is unknown, may be due to its ability to induce erythroid differentiation.

URLhttp://dx.plos.org/10.1371/journal.pmed.0050035
DOI10.1371/journal.pmed.0050035
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/18271621?dopt=Abstract

Alternate JournalPLoS Med.
PubMed ID18271621
PubMed Central IDPMC2235894
Grant ListK08 HL078818 / HL / NHLBI NIH HHS / United States
R01 HG003945 / HG / NHGRI NIH HHS / United States
1R01-HG003945-01 / HG / NHGRI NIH HHS / United States
K08-HL078818-01 / HL / NHLBI NIH HHS / United States