Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Antipova, AA, Stockwell, BR, Golub, TR |
Journal | Genome Biol |
Volume | 9 |
Issue | 3 |
Pages | R47 |
Date Published | 2008 |
ISSN | 1474-760X |
Keywords | Aurintricarboxylic Acid, Cell Line, Tumor, Drug Evaluation, Preclinical, Extracellular Signal-Regulated MAP Kinases, Gene Expression, Humans, Protein Kinase Inhibitors, Receptor, Platelet-Derived Growth Factor alpha, Receptor, Platelet-Derived Growth Factor beta, Signal Transduction, Structure-Activity Relationship |
Abstract | Here we describe a proof-of-concept experiment designed to explore the possibility of using gene expression-based high-throughput screening (GE-HTS) to find inhibitors of a signaling cascade, using platelet derived growth factor receptor (PDGFR) signaling as the example. The previously unrecognized ability of aurintricarboxylic acid to inhibit PDGFR signaling, discovered through a screen of 1,739 compounds, demonstrates the feasibility and generalizability of GE-HTS for the discovery of small molecule modulators of any signaling pathway of interest. |
URL | http://genomebiology.com/content/9/3/R47 |
DOI | 10.1186/gb-2008-9-3-r47 |
Pubmed | |
Alternate Journal | Genome Biol. |
PubMed ID | 18312689 |
PubMed Central ID | PMC2397499 |
Grant List | R01 CA097061 / CA / NCI NIH HHS / United States CA97061 / CA / NCI NIH HHS / United States / / Howard Hughes Medical Institute / United States |
Genome Biol DOI:10.1186/gb-2008-9-3-r47
Gene expression-based screening for inhibitors of PDGFR signaling.
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