Using CellProfiler for automatic identification and measurement of biological objects in images.

Curr Protoc Mol Biol
Authors
Keywords
Abstract

Visual analysis is required to perform many biological experiments, from counting yeast colonies to measuring the size and shape of individual cells or the intensity of fluorescently labeled proteins within them. This unit outlines the use of CellProfiler, a free, open-source image analysis tool that extracts quantitative information from biological images. It includes a step-by-step protocol for automated analysis of the number, color, and size of yeast colonies growing on agar plates, but the methods can be adapted to identify and measure any objects in images. The flexibility of the software allows users to tailor pipelines of adjustable modules to fit different biological experiments, to generate accurate measurements from dozens or even hundreds of thousands of images.

Year of Publication
2008
Journal
Curr Protoc Mol Biol
Volume
Chapter 14
Pages
Unit 14.17
Date Published
2008 Apr
ISSN
1934-3647
URL
DOI
10.1002/0471142727.mb1417s82
PubMed ID
18425761
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