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Development DOI:10.1242/dev.023200

In vivo birthdating by BAPTISM reveals that trigeminal sensory neuron diversity depends on early neurogenesis.

Publication TypeJournal Article
Year of Publication2008
AuthorsCaron, SJC, Prober, D, Choy, M, Schier, AF
Date Published2008 Oct
KeywordsAnimals, Animals, Genetically Modified, Gene Expression Regulation, Developmental, Ion Channels, Luminescent Proteins, Models, Neurological, Neurogenesis, Recombinant Proteins, Sensory Receptor Cells, Time Factors, Transient Receptor Potential Channels, Trigeminal Ganglion, Zebrafish, Zebrafish Proteins

Among sensory systems, the somatic sense is exceptional in its ability to detect a wide range of chemical, mechanical and thermal stimuli. How this sensory diversity is established during development remains largely elusive. We devised a method (BAPTISM) that uses the photoconvertible fluorescent protein Kaede to simultaneously analyze birthdate and cell fate in live zebrafish embryos. We found that trigeminal sensory ganglia are formed from early-born and late-born neurons. Early-born neurons give rise to multiple classes of sensory neurons that express different ion channels. By contrast, late-born neurons are restricted in their fate and do not form chemosensory neurons expressing the ion channel TrpA1b. Accordingly, larvae lacking early-born neurons do not respond to the TrpA1b agonist allyl isothiocyanate. These results indicate that the multimodal specification and function of trigeminal sensory ganglia depends on the timing of neurogenesis.


Alternate JournalDevelopment
PubMed ID18755773
PubMed Central IDPMC2596952
Grant ListR01 NS049319-03 / NS / NINDS NIH HHS / United States
K99 NS060996-01 / NS / NINDS NIH HHS / United States
K99 NS060996 / NS / NINDS NIH HHS / United States
R01 NS049319 / NS / NINDS NIH HHS / United States
R01 NS049319-02 / NS / NINDS NIH HHS / United States
R01 NS049319-01 / NS / NINDS NIH HHS / United States
R01 NS049319-04 / NS / NINDS NIH HHS / United States