|Publication Type||Journal Article|
|Year of Publication||2009|
|Authors||Nusbaum, C, Ohsumi, TK, Gomez, J, Aquadro, J, Victor, TC, Warren, RM, Hung, DT, Birren, BW, Lander, ES, Jaffe, DB|
Our variant ascertainment algorithm, VAAL, uses massively parallel DNA sequence data to identify differences between bacterial genomes with high sensitivity and specificity. VAAL detected approximately 98% of differences (including large insertion-deletions) between pairs of strains from three species while calling no false positives. VAAL also pinpointed a single mutation between Vibrio cholerae genomes, identifying an antibiotic's site of action by identifying sequence differences between drug-sensitive strains and drug-resistant derivatives.