Sensitive, specific polymorphism discovery in bacteria using massively parallel sequencing.
Nat Methods
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Abstract | Our variant ascertainment algorithm, VAAL, uses massively parallel DNA sequence data to identify differences between bacterial genomes with high sensitivity and specificity. VAAL detected approximately 98% of differences (including large insertion-deletions) between pairs of strains from three species while calling no false positives. VAAL also pinpointed a single mutation between Vibrio cholerae genomes, identifying an antibiotic's site of action by identifying sequence differences between drug-sensitive strains and drug-resistant derivatives. |
Year of Publication | 2009
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Journal | Nat Methods
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Volume | 6
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Issue | 1
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Pages | 67-9
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Date Published | 2009 Jan
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ISSN | 1548-7105
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URL | |
DOI | 10.1038/nmeth.1286
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PubMed ID | 19079253
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PubMed Central ID | PMC2613166
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Grant list | R01 HG003474-03 / HG / NHGRI NIH HHS / United States
R01 HG003474 / HG / NHGRI NIH HHS / United States
R01 HG003474-02 / HG / NHGRI NIH HHS / United States
R01 HG003474-01A1 / HG / NHGRI NIH HHS / United States
R01 HG003474-04 / HG / NHGRI NIH HHS / United States
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