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Curr Protoc Hum Genet DOI:10.1002/0471142905.hg0212s60

SNP genotyping using the Sequenom MassARRAY iPLEX platform.

Publication TypeJournal Article
Year of Publication2009
AuthorsGabriel, S, Ziaugra, L, Tabbaa, D
JournalCurr Protoc Hum Genet
VolumeChapter 2
PagesUnit 2.12
Date Published2009 Jan
ISSN1934-8258
KeywordsAlleles, DNA Primers, Genotype, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Abstract

The method for SNP genotyping described in this unit is based on the commercially available Sequenom MassARRAY platform. The assay consists of an initial locus-specific PCR reaction, followed by single base extension using mass-modified dideoxynucleotide terminators of an oligonucleotide primer which anneals immediately upstream of the polymorphic site of interest. Using MALDI-TOF mass spectrometry, the distinct mass of the extended primer identifies the SNP allele.

URLhttp://dx.doi.org/10.1002/0471142905.hg0212s60
DOI10.1002/0471142905.hg0212s60
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/19170031?dopt=Abstract

Alternate JournalCurr Protoc Hum Genet
PubMed ID19170031