SNP genotyping using the Sequenom MassARRAY iPLEX platform.

Curr Protoc Hum Genet
Authors
Keywords
Abstract

The method for SNP genotyping described in this unit is based on the commercially available Sequenom MassARRAY platform. The assay consists of an initial locus-specific PCR reaction, followed by single base extension using mass-modified dideoxynucleotide terminators of an oligonucleotide primer which anneals immediately upstream of the polymorphic site of interest. Using MALDI-TOF mass spectrometry, the distinct mass of the extended primer identifies the SNP allele.

Year of Publication
2009
Journal
Curr Protoc Hum Genet
Volume
Chapter 2
Pages
Unit 2.12
Date Published
2009 Jan
ISSN
1934-8258
URL
DOI
10.1002/0471142905.hg0212s60
PubMed ID
19170031
Links