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Curr Fungal Infect Rep DOI:10.1007/s12281-017-0276-7

Harnessing Whole Genome Sequencing in Medical Mycology.

Publication TypeJournal Article
Year of Publication2017
AuthorsCuomo, CA
JournalCurr Fungal Infect Rep
Volume11
Issue2
Pages52-59
Date Published2017
ISSN1936-3761
Abstract

PURPOSE OF REVIEW: Comparative genome sequencing studies of human fungal pathogens enable identification of genes and variants associated with virulence and drug resistance. This review describes current approaches, resources, and advances in applying whole genome sequencing to study clinically important fungal pathogens.RECENT FINDINGS: Genomes for some important fungal pathogens were only recently assembled, revealing gene family expansions in many species and extreme gene loss in one obligate species. The scale and scope of species sequenced is rapidly expanding, leveraging technological advances to assemble and annotate genomes with higher precision. By using iteratively improved reference assemblies or those generated de novo for new species, recent studies have compared the sequence of isolates representing populations or clinical cohorts. Whole genome approaches provide the resolution necessary for comparison of closely related isolates, for example, in the analysis of outbreaks or sampled across time within a single host.SUMMARY: Genomic analysis of fungal pathogens has enabled both basic research and diagnostic studies. The increased scale of sequencing can be applied across populations, and new metagenomic methods allow direct analysis of complex samples.

DOI10.1007/s12281-017-0276-7
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/28904649?dopt=Abstract

Alternate JournalCurr Fungal Infect Rep
PubMed ID28904649
PubMed Central IDPMC5570814
Grant ListU19 AI110818 / AI / NIAID NIH HHS / United States