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Mem Inst Oswaldo Cruz DOI:10.1590/0074-02760170473

Advances in Cryptococcus genomics: insights into the evolution of pathogenesis.

Publication TypeJournal Article
Year of Publication2018
AuthorsCuomo, CA, Rhodes, J, Desjardins, CA
JournalMem Inst Oswaldo Cruz
Date Published2018
KeywordsCryptococcus gattii, Cryptococcus neoformans, DNA, Fungal, Genetic Variation, Genotype, Multilocus Sequence Typing, Phylogeny, Phylogeography

Cryptococcus species are the causative agents of cryptococcal meningitis, a significant source of mortality in immunocompromised individuals. Initial work on the molecular epidemiology of this fungal pathogen utilized genotyping approaches to describe the genetic diversity and biogeography of two species, Cryptococcus neoformans and Cryptococcus gattii. Whole genome sequencing of representatives of both species resulted in reference assemblies enabling a wide array of downstream studies and genomic resources. With the increasing availability of whole genome sequencing, both species have now had hundreds of individual isolates sequenced, providing fine-scale insight into the evolution and diversification of Cryptococcus and allowing for the first genome-wide association studies to identify genetic variants associated with human virulence. Sequencing has also begun to examine the microevolution of isolates during prolonged infection and to identify variants specific to outbreak lineages, highlighting the potential role of hyper-mutation in evolving within short time scales. We can anticipate that further advances in sequencing technology and sequencing microbial genomes at scale, including metagenomics approaches, will continue to refine our view of how the evolution of Cryptococcus drives its success as a pathogen.


Alternate JournalMem. Inst. Oswaldo Cruz
PubMed ID29513784
PubMed Central IDPMC5851040
Grant ListU19 AI110818 / AI / NIAID NIH HHS / United States