|Publication Type||Journal Article|
|Year of Publication||2009|
|Authors||W An, F, Tolliday, NJ|
|Journal||Methods Mol Biol|
|Keywords||Animals, Biological Assay, Cells, Combinatorial Chemistry Techniques, Drug Evaluation, Preclinical, Humans, Small Molecule Libraries|
Cell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review the history and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS, drawing upon themes that will reemerge in subsequent chapters in this book. Approaches for successful experimental design and execution of cell-based HTS are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques described in this book.
|Alternate Journal||Methods Mol. Biol.|