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Methods Mol Biol DOI:10.1007/978-1-60327-545-3_1

Introduction: cell-based assays for high-throughput screening.

Publication TypeJournal Article
Year of Publication2009
AuthorsW An, F, Tolliday, NJ
JournalMethods Mol Biol
Date Published2009
KeywordsAnimals, Biological Assay, Cells, Combinatorial Chemistry Techniques, Drug Evaluation, Preclinical, Humans, Small Molecule Libraries

Cell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review the history and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS, drawing upon themes that will reemerge in subsequent chapters in this book. Approaches for successful experimental design and execution of cell-based HTS are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques described in this book.


Alternate JournalMethods Mol. Biol.
PubMed ID19347612