Scientific Publications

High-throughput real-time PCR for detection of gene-expression levels.

Publication TypeJournal Article
AuthorsWagner, BK, and Arany Z.
AbstractWhile many high-throughput screening campaigns involve the measurement of protein levels or locations, at times it is desirable to measure the levels of gene expression in response to small molecules. Here, we describe a method for capturing mRNA in multiwell plates following compound treatment and measuring gene expression using real-time PCR. This streamlined protocol provides complementary information to conventional phenotypic cell-based assays, and is especially useful in cases where the gene of interest is thought to serve a regulatory function in downstream cellular phenotypes.
Year of Publication2012
JournalMethods in molecular biology (Clifton, N.J.)
Volume486
Pages167-75
Date Published (YYYY/MM/DD)2012/01/09
ISSN Number1064-3745
DOI10.1007/978-1-60327-545-3_12
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/19347623?dopt=Abstract