Chromatin Accessibility Impacts Transcriptional Reprogramming in Oocytes.
Oocytes have a remarkable ability to reactivate silenced genes in somatic cells. However, it is not clear how the chromatin architecture of somatic cells affects this transcriptional reprogramming. Here, we investigated the relationship between the chromatin opening and transcriptional activation. We reveal changes in chromatin accessibility and their relevance to transcriptional reprogramming after transplantation of somatic nuclei into Xenopus oocytes. Genes that are silenced, but have pre-existing open transcription start sites in donor cells, are prone to be activated after nuclear transfer, suggesting that the chromatin signature of somatic nuclei influences transcriptional reprogramming. There are also activated genes associated with new open chromatin sites, and transcription factors in oocytes play an important role in transcriptional reprogramming from such genes. Finally, we show that genes resistant to reprogramming are associated with closed chromatin configurations. We conclude that chromatin accessibility is a central factor for successful transcriptional reprogramming in oocytes.
|Year of Publication||
2018 07 10
|PubMed Central ID||
MR/K011022/1 / MRC_ / Medical Research Council / United Kingdom
Wellcome Trust / United Kingdom
MR/L023784/2 / MRC_ / Medical Research Council / United Kingdom
U41 HG007000 / HG / NHGRI NIH HHS / United States
MR/L023784/1 / MRC_ / Medical Research Council / United Kingdom
R01 GM113708 / GM / NIGMS NIH HHS / United States