|Publication Type||Journal Article|
|Year of Publication||2009|
|Authors||Garber, M, Zody, MC, Arachchi, HM, Berlin, A, Gnerre, S, Green, LM, Lennon, N, Nusbaum, C|
The most recent release of the finished human genome contains 260 euchromatic gaps (excluding chromosome Y). Recent work has helped explain a large number of these unresolved regions as 'structural' in nature. Another class of gaps is likely to be refractory to clone-based approaches, and cannot be approached in ways previously described. We present an approach for closing these gaps using 454 sequencing. As a proof of principle, we closed all three remaining non-structural gaps in chromosome 15.