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Genome Biol DOI:10.1186/gb-2009-10-6-r60

Closing gaps in the human genome using sequencing by synthesis.

Publication TypeJournal Article
Year of Publication2009
AuthorsGarber, M, Zody, MC, Arachchi, HM, Berlin, A, Gnerre, S, Green, LM, Lennon, N, Nusbaum, C
JournalGenome Biol
Volume10
Issue6
PagesR60
Date Published2009
ISSN1474-760X
KeywordsBase Sequence, Cell Line, Chromosomes, Human, Pair 15, Genome, Human, Humans, Sequence Analysis, DNA
Abstract

The most recent release of the finished human genome contains 260 euchromatic gaps (excluding chromosome Y). Recent work has helped explain a large number of these unresolved regions as 'structural' in nature. Another class of gaps is likely to be refractory to clone-based approaches, and cannot be approached in ways previously described. We present an approach for closing these gaps using 454 sequencing. As a proof of principle, we closed all three remaining non-structural gaps in chromosome 15.

URLhttp://genomebiology.com/content/10/6/R60
DOI10.1186/gb-2009-10-6-r60
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/19490611?dopt=Abstract

Alternate JournalGenome Biol.
PubMed ID19490611
PubMed Central IDPMC2718494