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Cell DOI:10.1016/j.cell.2018.07.028

Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain.

Publication TypeJournal Article
Year of Publication2018
AuthorsSaunders, A, Macosko, EZ, Wysoker, A, Goldman, M, Krienen, FM, de Rivera, H, Bien, E, Baum, M, Bortolin, L, Wang, S, Goeva, A, Nemesh, J, Kamitaki, N, Brumbaugh, S, Kulp, D, McCarroll, SA
JournalCell
Volume174
Issue4
Pages1015-1030.e16
Date Published2018 08 09
ISSN1097-4172
Abstract

The mammalian brain is composed of diverse, specialized cell populations. To systematically ascertain and learn from these cellular specializations, we used Drop-seq to profile RNA expression in 690,000 individual cells sampled from 9 regions of the adult mouse brain. We identified 565 transcriptionally distinct groups of cells using computational approaches developed to distinguish biological from technical signals. Cross-region analysis of these 565 cell populations revealed features of brain organization, including a gene-expression module for synthesizing axonal and presynaptic components, patterns in the co-deployment of voltage-gated ion channels, functional distinctions among the cells of the vasculature and specialization of glutamatergic neurons across cortical regions. Systematic neuronal classifications for two complex basal ganglia nuclei and the striatum revealed a rare population of spiny projection neurons. This adult mouse brain cell atlas, accessible through interactive online software (DropViz), serves as a reference for development, disease, and evolution.

DOI10.1016/j.cell.2018.07.028
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/30096299?dopt=Abstract

Alternate JournalCell
PubMed ID30096299
PubMed Central IDPMC6447408
Grant ListT32 GM007753 / GM / NIGMS NIH HHS / United States
T32 MH020017 / MH / NIMH NIH HHS / United States