A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers.
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Abstract | There is a need for large-scale, longitudinal studies to determine the mechanisms by which the gut microbiome and its interactions with the host affect human health and disease. Current methods for profiling the microbiome typically utilize next-generation sequencing applications that are expensive, slow, and complex. Here, we present a synthetic biology platform for affordable, on-demand, and simple analysis of microbiome samples using RNA toehold switch sensors in paper-based, cell-free reactions. We demonstrate species-specific detection of mRNAs from 10 different bacteria that affect human health and four clinically relevant host biomarkers. We develop a method to quantify mRNA using our toehold sensors and validate our platform on clinical stool samples by comparison to RT-qPCR. We further highlight the potential clinical utility of the platform by showing that it can be used to rapidly and inexpensively detect toxin mRNA in the diagnosis of Clostridium difficile infections. |
Year of Publication | 2018
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Journal | Nat Commun
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Volume | 9
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Issue | 1
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Pages | 3347
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Date Published | 2018 08 21
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ISSN | 2041-1723
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DOI | 10.1038/s41467-018-05864-4
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PubMed ID | 30131493
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PubMed Central ID | PMC6104080
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Grant list | K23 DK097142 / DK / NIDDK NIH HHS / United States
P30 DK043351 / DK / NIDDK NIH HHS / United States
R03 DK112909 / DK / NIDDK NIH HHS / United States
T32 DK007191 / DK / NIDDK NIH HHS / United States
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