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PLoS One DOI:10.1371/journal.pone.0006143

microRNA expression during trophectoderm specification.

Publication TypeJournal Article
Year of Publication2009
AuthorsViswanathan, SR, Mermel, CH, Lu, J, Lu, C-W, Golub, TR, Daley, GQ
JournalPLoS One
Date Published2009 Jul 03
KeywordsAnimals, Blastocyst, Cell Differentiation, Cell Lineage, Ectoderm, Mice, MicroRNAs, Pluripotent Stem Cells

BACKGROUND: Segregation of the trophectoderm from the inner cell mass of the embryo represents the first cell-fate decision of mammalian development. Transcription factors essential for specifying trophectoderm have been identified, but the role of microRNAs (miRNAs) in modulating this fate-choice has been largely unexplored. We have compared miRNA expression in embryonic stem cell (ESC)-derived trophectoderm and in staged murine embryos to identify a set of candidate miRNAs likely to be involved in trophectoderm specification.

RESULTS: We profiled embryonic stem cells (ESCs) as they were induced to differentiate into trophectodermal cells by ectopic expression of HRas/Q61L. We also profiled murine embryos at progressive stages of preimplantation development (zygote, 2-cell, 4-cell, 8-cell, morula, and blastocyst), which includes the time window in which the trophectoderm is specified in vivo Q61L/H.

CONCLUSIONS: We describe miRNA expression changes that occur during trophectoderm specification and validate that our in vitro system faithfully recapitulates trophectoderm specification in vivo. By comparing our in vitro and in vivo datasets, we have identified a minimal set of candidate miRNAs likely to play a role in trophectoderm specification. These miRNAs are predicted to regulate a host of development-associated target genes, and many of these miRNAs have previously reported roles in development and differentiation. Additionally, we highlight a number of miRNAs whose tight developmental regulation may reflect a functional role in other stages of embryogenesis. Our embryo profiling data may be useful to investigators studying trophectoderm specification and other stages of preimplantation development.


Alternate JournalPLoS ONE
PubMed ID19582159
PubMed Central IDPMC2702083
Grant ListT32 GM007753 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States