|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Levin, JZ, Berger, MF, Adiconis, X, Rogov, P, Melnikov, A, Fennell, T, Nusbaum, C, Garraway, LA, Gnirke, A|
Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes.