You are here

Nature methods DOI:10.1038/nmeth.1404

Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.

Publication TypeJournal Article
Year of Publication2010
AuthorsGoren, A, Ozsolak, F, Shoresh, N, Ku, M, Adli, M, Hart, C, Gymrek, M, Zuk, O, Regev, A, Milos, PM, Bernstein, BE
JournalNature methods
Volume7
Issue1
Pages47-9
Date Published2010/01/01
ISSN1548-7091
Abstract

Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations.

URLhttp://dx.doi.org/10.1038/nmeth.1404
DOI10.1038/nmeth.1404
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/19946276?dopt=Abstract