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Nat Methods DOI:10.1038/nmeth.1404

Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA.

Publication TypeJournal Article
Year of Publication2010
AuthorsGoren, A, Ozsolak, F, Shoresh, N, Ku, M, Adli, M, Hart, C, Gymrek, M, Zuk, O, Regev, A, Milos, PM, Bernstein, BE
JournalNat Methods
Volume7
Issue1
Pages47-9
Date Published2010 Jan
ISSN1548-7105
KeywordsAnimals, Bias (Epidemiology), Chromatin, Chromatin Immunoprecipitation, Gene Expression Profiling, Genome, Humans, Mice, Sequence Analysis, DNA, Transcription Factors
Abstract

Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations.

URLhttp://dx.doi.org/10.1038/nmeth.1404
DOI10.1038/nmeth.1404
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/19946276?dopt=Abstract

Alternate JournalNat. Methods
PubMed ID19946276
PubMed Central IDPMC2862482
Grant ListR01 HG004401 / HG / NHGRI NIH HHS / United States
U54 HG004570-02 / HG / NHGRI NIH HHS / United States
R01 HG004401-03 / HG / NHGRI NIH HHS / United States
U54 HG004570-01 / HG / NHGRI NIH HHS / United States
R44 HG005279-01 / HG / NHGRI NIH HHS / United States
U54 HG004570 / HG / NHGRI NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
R44 HG005279 / HG / NHGRI NIH HHS / United States
U54 HG004570-03 / HG / NHGRI NIH HHS / United States
DP1 OD003958 / OD / NIH HHS / United States