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Nat Biotechnol DOI:10.1038/nbt.4239

Intron retention is a source of neoepitopes in cancer.

Publication TypeJournal Article
Year of Publication2018
AuthorsSmart, AC, Margolis, CA, Pimentel, H, He, MXiao, Miao, D, Adeegbe, D, Fugmann, T, Wong, K-K, Van Allen, EM
JournalNat Biotechnol
Date Published2018 12
KeywordsCancer Vaccines, Cell Line, Tumor, Computer Simulation, Epitope Mapping, Epitopes, Humans, Introns, Models, Genetic, Neoplasms, RNA

We present an in silico approach to identifying neoepitopes derived from intron retention events in tumor transcriptomes. Using mass spectrometry immunopeptidome analysis, we show that retained intron neoepitopes are processed and presented on MHC I on the surface of cancer cell lines. RNA-derived neoepitopes should be considered for prospective personalized cancer vaccine development.


Alternate JournalNat Biotechnol
PubMed ID30114007
PubMed Central IDPMC6226333
Grant ListK08 CA188615 / CA / NCI NIH HHS / United States
R01 CA166480 / CA / NCI NIH HHS / United States
R01 CA227388 / CA / NCI NIH HHS / United States