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Clinical chemistry DOI:10.1373/clinchem.2009.138420

Automated detection of inaccurate and imprecise transitions in peptide quantification by multiple reaction monitoring mass spectrometry.

Publication TypeJournal Article
Year of Publication2010
AuthorsAbbatiello, SE, Mani, DR, Keshishian, H, Carr, SA
JournalClinical chemistry
Volume56
Issue2
Pages291-305
Date Published2010/02/01
ISSN0009-9147
Abstract

Multiple reaction monitoring mass spectrometry (MRM-MS) of peptides with stable isotope-labeled internal standards (SISs) is increasingly being used to develop quantitative assays for proteins in complex biological matrices. These assays can be highly precise and quantitative, but the frequent occurrence of interferences requires that MRM-MS data be manually reviewed, a time-intensive process subject to human error. We developed an algorithm that identifies inaccurate transition data based on the presence of interfering signal or inconsistent recovery among replicate samples.

URLhttp://www.clinchem.org/cgi/pmidlookup?view=long&pmid=20022980
DOI10.1373/clinchem.2009.138420
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/20022980?dopt=Abstract