|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Gu, H, Bock, C, Mikkelsen, TS, Jäger, N, Smith, ZD, Tomazou, E, Gnirke, A, Lander, ES, Meissner, A|
Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples.