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Nature methods DOI:10.1038/nmeth.1414

Genome-scale DNA methylation mapping of clinical samples at single-nucleotide resolution.

Publication TypeJournal Article
Year of Publication2010
AuthorsGu, H, Bock, C, Mikkelsen, TS, Jäger, N, Smith, ZD, Tomazou, E, Gnirke, A, Lander, ES, Meissner, A
JournalNature methods
Volume7
Issue2
Pages133-6
Date Published2010/02/01
ISSN1548-7091
Abstract

Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples.

URLhttp://dx.doi.org/10.1038/nmeth.1414
DOI10.1038/nmeth.1414
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/20062050?dopt=Abstract