|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Lennon, NJ, Lintner, RE, Anderson, S, Alvarez, P, Barry, A, Brockman, W, Daza, R, Erlich, RL, Giannoukos, G, Green, L, Hollinger, A, Hoover, CA, Jaffe, DB, Juhn, F, McCarthy, D, Perrin, D, Ponchner, K, Powers, TL, Rizzolo, K, Robbins, D, Ryan, E, Russ, C, Sparrow, T, Stalker, J, Steelman, S, Weiand, M, Zimmer, A, Henn, MR, Nusbaum, C, Nicol, R|
We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method.