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Genome Biol DOI:10.1186/gb-2010-11-2-r15

A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454.

Publication TypeJournal Article
Year of Publication2010
AuthorsLennon, NJ, Lintner, RE, Anderson, S, Alvarez, P, Barry, A, Brockman, W, Daza, R, Erlich, RL, Giannoukos, G, Green, L, Hollinger, A, Hoover, CA, Jaffe, DB, Juhn, F, McCarthy, D, Perrin, D, Ponchner, K, Powers, TL, Rizzolo, K, Robbins, D, Ryan, E, Russ, C, Sparrow, T, Stalker, J, Steelman, S, Weiand, M, Zimmer, A, Henn, MR, Nusbaum, C, Nicol, R
JournalGenome Biol
Date Published2010
KeywordsAlgorithms, Automatic Data Processing, Gene Library, High-Throughput Screening Assays, Humans, Microspheres, Sequence Analysis, DNA

We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method.


Alternate JournalGenome Biol.
PubMed ID20137071
PubMed Central IDPMC2872875