You are here

PloS one DOI:10.1371/journal.pone.0009083

Analysis of high-throughput sequencing and annotation strategies for phage genomes.

Publication TypeJournal Article
Year of Publication2010
AuthorsHenn, MR, Sullivan, MB, Stange-Thomann, N, Osburne, MS, Berlin, AM, Kelly, L, Yandava, C, Kodira, C, Zeng, Q, Weiand, M, Sparrow, T, Saif, S, Giannoukos, G, Young, SK, Nusbaum, C, Birren, BW, Chisholm, SW
JournalPloS one
Date Published2010/02/05

Bacterial viruses (phages) play a critical role in shaping microbial populations as they influence both host mortality and horizontal gene transfer. As such, they have a significant impact on local and global ecosystem function and human health. Despite their importance, little is known about the genomic diversity harbored in phages, as methods to capture complete phage genomes have been hampered by the lack of knowledge about the target genomes, and difficulties in generating sufficient quantities of genomic DNA for sequencing. Of the approximately 550 phage genomes currently available in the public domain, fewer than 5% are marine phage.