|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||W An, F, Tolliday, N|
|Date Published||2010 Jun|
|Keywords||Animals, Biological Assay, Cells, Combinatorial Chemistry Techniques, High-Throughput Screening Assays|
Cell-based assays represent approximately half of all high-throughput screens currently performed. Here, we review in brief the history and status of high-throughput screening (HTS), and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS. Approaches for successful experimental design and execution of cell-based screens are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques used.
|Alternate Journal||Mol. Biotechnol.|
|Grant List||N01-CO-12400 / CO / NCI NIH HHS / United States|