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Molecular biotechnology DOI:10.1007/s12033-010-9251-z

Cell-based assays for high-throughput screening.

Publication TypeJournal Article
Year of Publication2010
AuthorsAn, WF, Tolliday, N
JournalMolecular biotechnology
Volume45
Issue2
Pages180-6
Date Published2010/06/01
ISSN1073-6085
Abstract

Cell-based assays represent approximately half of all high-throughput screens currently performed. Here, we review in brief the history and status of high-throughput screening (HTS), and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS. Approaches for successful experimental design and execution of cell-based screens are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques used.

URLhttp://dx.doi.org/10.1007/s12033-010-9251-z
DOI10.1007/s12033-010-9251-z
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/20151227?dopt=Abstract