Cell-based assays for high-throughput screening.

Mol Biotechnol
Authors
Keywords
Abstract

Cell-based assays represent approximately half of all high-throughput screens currently performed. Here, we review in brief the history and status of high-throughput screening (HTS), and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS. Approaches for successful experimental design and execution of cell-based screens are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques used.

Year of Publication
2010
Journal
Mol Biotechnol
Volume
45
Issue
2
Pages
180-6
Date Published
2010 Jun
ISSN
1559-0305
URL
DOI
10.1007/s12033-010-9251-z
PubMed ID
20151227
Links
Grant list
N01-CO-12400 / CO / NCI NIH HHS / United States