Scientific Publications

Toward a systems-level analysis of infection biology: a new method for conducting genetic screens in human cells.

Publication TypeJournal Article
AuthorsStanley, SA, and Hung DT
AbstractLoss-of-function genetic screens have facilitated great strides in our understanding of the biology of model organisms but have not been possible in diploid human cells. A recent report by Brummelkamp's group in Science describes the use of insertional mutagenesis to generate loss-of-function alleles in a largely haploid human cell line and demonstrates the versatility of this method in screens designed to investigate the host/pathogen interaction. This approach has strengths that are complementary to existing strategies and will facilitate progress toward a systems-level understanding of infectious disease and ultimately the development of new therapeutics.
Year of Publication2009
JournalScience translational medicine
Volume1
Issue11
Pages11ps13
Date Published (YYYY/MM/DD)2009/12/16
ISSN Number1946-6234
DOI10.1126/scitranslmed.3000550
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/20371455?dopt=Abstract