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Sci Transl Med DOI:10.1126/scitranslmed.3000550

Toward a systems-level analysis of infection biology: a new method for conducting genetic screens in human cells.

Publication TypeJournal Article
Year of Publication2009
AuthorsStanley, SA, Hung, DT
JournalSci Transl Med
Date Published2009 Dec 16
KeywordsAnimals, Genetic Testing, Host-Pathogen Interactions, Humans, Infection, Mutagenesis, Insertional, RNA, Small Interfering

Loss-of-function genetic screens have facilitated great strides in our understanding of the biology of model organisms but have not been possible in diploid human cells. A recent report by Brummelkamp's group in Science describes the use of insertional mutagenesis to generate loss-of-function alleles in a largely haploid human cell line and demonstrates the versatility of this method in screens designed to investigate the host/pathogen interaction. This approach has strengths that are complementary to existing strategies and will facilitate progress toward a systems-level understanding of infectious disease and ultimately the development of new therapeutics.


Alternate JournalSci Transl Med
PubMed ID20371455
Grant List / / Intramural NIH HHS / United States