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Nat Biotechnol DOI:10.1038/nbt.1632

Dynamic single-cell imaging of direct reprogramming reveals an early specifying event.

Publication TypeJournal Article
Year of Publication2010
AuthorsSmith, ZD, Nachman, I, Regev, A, Meissner, A
JournalNat Biotechnol
Date Published2010 May
KeywordsAnimals, Cell Proliferation, Cells, Cultured, Cellular Reprogramming, Embryonic Stem Cells, Fibroblasts, Gene Knockdown Techniques, Immunohistochemistry, Mice, Microscopy, Fluorescence, Models, Biological, Photography, Tumor Suppressor Protein p53

The study of induced pluripotency often relies on experimental approaches that average measurements across a large population of cells, the majority of which do not become pluripotent. Here we used high-resolution, time-lapse imaging to trace the reprogramming process over 2 weeks from single mouse embryonic fibroblasts (MEFs) to pluripotency factor-positive colonies. This enabled us to calculate a normalized cell-of-origin reprogramming efficiency that takes into account only the initial MEFs that respond to form reprogrammed colonies rather than the larger number of final colonies. Furthermore, this retrospective analysis revealed that successfully reprogramming cells undergo a rapid shift in their proliferative rate that coincides with a reduction in cellular area. This event occurs as early as the first cell division and with similar kinetics in all cells that form induced pluripotent stem (iPS) cell colonies. These data contribute to the theoretical modeling of reprogramming and suggest that certain parts of the reprogramming process follow defined rather than stochastic steps.


Alternate JournalNat. Biotechnol.
PubMed ID20436460
PubMed Central IDPMC2908494
Grant List / / Howard Hughes Medical Institute / United States
DP1 OD003958 / OD / NIH HHS / United States
DP1 OD003958-04 / OD / NIH HHS / United States