Scientific Publications

Screening cellular feature measurements for image-based assay development.

Publication TypeJournal Article
AuthorsLogan, DJ, and Carpenter AE
AbstractThe typical "design" approach to image-based assay development involves choosing measurements that are likely to correlate with the phenotype of interest, based on the researcher's intuition and knowledge of image analysis. An alternate "screening" approach is to measure a large number of cellular features and systematically test each feature to identify those that are best able to distinguish positive and negative controls while taking precautions to avoid overfitting the available data. The cell measurement software the authors previously developed, CellProfiler, makes both approaches straightforward, easing the process of assay development. Here, they demonstrate the use of the screening approach to image assay development to select the best measures for scoring publicly available image sets of 2 cytoplasm-to-nucleus translocation assays and 2 Transfluor assays. The authors present the resulting assay quality measures as a baseline for future algorithm comparisons, and all software, methods, and images they present are freely available.
Year of Publication2010
JournalJournal of biomolecular screening
Volume15
Issue7
Pages840-6
Date Published (YYYY/MM/DD)2010/08/01
ISSN Number1087-0571
DOI10.1177/1087057110370895
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/20516293?dopt=Abstract