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Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.] DOI:10.1002/0471142905.hg1804s66

Targeted exon sequencing by in-solution hybrid selection.

Publication TypeJournal Article
Year of Publication2010
AuthorsBlumenstiel, B, Cibulskis, K, Fisher, S, DeFelice, M, Barry, A, Fennell, T, Abreu, J, Minie, B, Costello, M, Young, G, Maquire, J, Kernytsky, A, Melnikov, A, Rogov, P, Gnirke, A, Gabriel, S
JournalCurrent protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
VolumeChapter 18
PagesUnit 18.4
Date Published2010/07/01
ISSN1934-8266
Abstract

This unit describes a protocol for the targeted enrichment of exons from randomly sheared genomic DNA libraries using an in-solution hybrid selection approach for sequencing on an Illumina Genome Analyzer II. The steps for designing and ordering a hybrid selection oligo pool are reviewed, as are critical steps for performing the preparation and hybrid selection of an Illumina paired-end library. Critical parameters, performance metrics, and analysis workflow are discussed.

URLhttp://dx.doi.org/10.1002/0471142905.hg1804s66
DOI10.1002/0471142905.hg1804s66
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/20582916?dopt=Abstract