Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors.
Authors | |
Keywords | |
Abstract | Dendritic cells (DCs) and monocytes play a central role in pathogen sensing, phagocytosis, and antigen presentation and consist of multiple specialized subtypes. However, their identities and interrelationships are not fully understood. Using unbiased single-cell RNA sequencing (RNA-seq) of ~2400 cells, we identified six human DCs and four monocyte subtypes in human blood. Our study reveals a new DC subset that shares properties with plasmacytoid DCs (pDCs) but potently activates T cells, thus redefining pDCs; a new subdivision within the CD1Csubset of DCs; the relationship between blastic plasmacytoid DC neoplasia cells and healthy DCs; and circulating progenitor of conventional DCs (cDCs). Our revised taxonomy will enable more accurate functional and developmental analyses as well as immune monitoring in health and disease. |
Year of Publication | 2017
|
Journal | Science
|
Volume | 356
|
Issue | 6335
|
Date Published | 2017 04 21
|
ISSN | 1095-9203
|
DOI | 10.1126/science.aah4573
|
PubMed ID | 28428369
|
PubMed Central ID | PMC5775029
|
Links | |
Grant list | T32 HG002295 / HG / NHGRI NIH HHS / United States
DP2 HG009623 / HG / NHGRI NIH HHS / United States
Howard Hughes Medical Institute / United States
P50 HG006193 / HG / NHGRI NIH HHS / United States
Wellcome Trust / United Kingdom
MR/N005872/1 / Medical Research Council / United Kingdom
P30 AI060354 / AI / NIAID NIH HHS / United States
RM1 HG006193 / HG / NHGRI NIH HHS / United States
|