Scientific Publications

A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries.

Publication TypeJournal Article
AuthorsFisher, S., Barry A., Abreu J., Minie B., Nolan J., Delorey TM, Young G., Fennell TJ, Allen A., Ambrogio L., Berlin AM, Blumenstiel B., Cibulskis K., Friedrich D., Johnson R., Juhn F., Reilly B., Shammas R., Stalker J., Sykes SM, Thompson J., Walsh J., Zimmer A., Zwirko Z., Gabriel S., Nicol R., and Nusbaum C.
AbstractGenome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.
Year of Publication2012
JournalGenome biology
Date Published (YYYY/MM/DD)2012/01/09
ISSN Number1465-6906