|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Fisher, S, Barry, A, Abreu, J, Minie, B, Nolan, J, Delorey, TM, Young, G, Fennell, TJ, Allen, A, Ambrogio, L, Berlin, AM, Blumenstiel, B, Cibulskis, K, Friedrich, D, Johnson, R, Juhn, F, Reilly, B, Shammas, R, Stalker, J, Sykes, SM, Thompson, J, Walsh, J, Zimmer, A, Zwirko, Z, Gabriel, S, Nicol, R, Nusbaum, C|
Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.