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Next-generation sequencing for HLA typing of class I loci.
|Publication Type||Journal Article|
|Authors||Erlich, RL, Jia X., Anderson S., Banks E., Gao X., Carrington M., Gupta N., DePristo MA, Henn MR, Lennon NJ, and de Bakker PI|
|Abstract||Comprehensive sequence characterization across the MHC is important for successful organ transplantation and genetic association studies. To this end, we have developed an automated sample preparation, molecular barcoding and multiplexing protocol for the amplification and sequence-determination of class I HLA loci. We have coupled this process to a novel HLA calling algorithm to determine the most likely pair of alleles at each locus.|
|Year of Publication||2011|
|Date Published (YYYY/MM/DD)||2011/01/18|