Scientific Publications

A public genome-scale lentiviral expression library of human ORFs.

Publication TypeJournal Article
AuthorsYang, X., Boehm JS, Yang X., Salehi-Ashtiani K., Hao T., Shen Y., Lubonja R., Thomas SR, Alkan O., Bhimdi T., Green TM, Johannessen CM, Silver SJ, Nguyen C., Murray RR, Hieronymus H., Balcha D., Fan C., Lin C., Ghamsari L., Vidal M., Hahn WC, Hill DE, and Root DE
AbstractFunctional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.
Year of Publication2011
JournalNature methods
Date Published (YYYY/MM/DD)2011/06/26
ISSN Number1548-7091