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Nature methods DOI:10.1038/nmeth.1638

A public genome-scale lentiviral expression library of human ORFs.

Publication TypeJournal Article
Year of Publication2011
AuthorsYang, X, Boehm, JS, Yang, X, Salehi-Ashtiani, K, Hao, T, Shen, Y, Lubonja, R, Thomas, SR, Alkan, O, Bhimdi, T, Green, TM, Johannessen, CM, Silver, SJ, Nguyen, C, Murray, RR, Hieronymus, H, Balcha, D, Fan, C, Lin, C, Ghamsari, L, Vidal, M, Hahn, WC, Hill, DE, Root, DE
JournalNature methods
Volume8
Issue8
Pages659-61
Date Published2011/06/26
ISSN1548-7091
Abstract

Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.

URLhttp://dx.doi.org/10.1038/nmeth.1638
DOI10.1038/nmeth.1638
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/21706014?dopt=Abstract