Scientific Publications

Systematic discovery of TLR signaling components delineates viral-sensing circuits.

Publication TypeJournal Article
AuthorsChevrier, N., Mertins P., Artyomov MN, Shalek AK, Iannacone M., Ciaccio MF, Gat-Viks I., Tonti E., Degrace MM, Clauser KR, Garber M., Eisenhaure TM, Yosef N., Robinson J., Sutton A., Andersen MS, Root DE, von Andrian U., Jones RB, Park H., Carr SA, Regev A., Amit I., and Hacohen N.
AbstractDeciphering the signaling networks that underlie normal and disease processes remains a major challenge. Here, we report the discovery of signaling components involved in the Toll-like receptor (TLR) response of immune dendritic cells (DCs), including a previously unkown pathway shared across mammalian antiviral responses. By combining transcriptional profiling, genetic and small-molecule perturbations, and phosphoproteomics, we uncover 35 signaling regulators, including 16 known regulators, involved in TLR signaling. In particular, we find that Polo-like kinases (Plk) 2 and 4 are essential components of antiviral pathways in vitro and in vivo and activate a signaling branch involving a dozen proteins, among which is Tnfaip2, a gene associated with autoimmune diseases but whose role was unknown. Our study illustrates the power of combining systematic measurements and perturbations to elucidate complex signaling circuits and discover potential therapeutic targets.
Year of Publication2011
JournalCell
Volume147
Issue4
Pages853-67
Date Published (YYYY/MM/DD)2011/11/11
ISSN Number0092-8674
DOI10.1016/j.cell.2011.10.022
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/22078882?dopt=Abstract