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Nat Methods DOI:10.1038/s41592-018-0007-1

Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling.

Publication TypeJournal Article
Year of Publication2018
AuthorsMyers, SA, Wright, J, Peckner, R, Kalish, BT, Zhang, F, Carr, SA
JournalNat Methods
Date Published2018 May 07
ISSN1548-7105
Abstract

Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.

DOI10.1038/s41592-018-0007-1
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/29735997?dopt=Abstract

Alternate JournalNat. Methods
PubMed ID29735997