Imaging mass spectrometry demonstrates age-related decline in human adipose plasticity.
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Abstract | Quantification of stable isotope tracers has revealed the dynamic state of living tissues. A new form of imaging mass spectrometry quantifies isotope ratios in domains much smaller than a cubic micron, enabling measurement of cell turnover and metabolism with stable isotope tracers at the single-cell level with a methodology we refer to as multi-isotope imaging mass spectrometry. In a first-in-human study, we utilize stable isotope tracers of DNA synthesis and de novo lipogenesis to prospectively measure cell birth and adipocyte lipid turnover. In a study of healthy adults, we elucidate an age-dependent decline in new adipocyte generation and adipocyte lipid turnover. A linear regression model suggests that the aging effect could be mediated by a decline in insulin-like growth factor-1 (IGF-1). This study therefore establishes a method for measurement of cell turnover and metabolism in humans with subcellular resolution while implicating the growth hormone/IGF-1 axis in adipose tissue aging. |
Year of Publication | 2017
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Journal | JCI Insight
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Volume | 2
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Issue | 5
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Pages | e90349
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Date Published | 2017 Mar 09
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ISSN | 2379-3708
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DOI | 10.1172/jci.insight.90349
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PubMed ID | 28289709
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PubMed Central ID | PMC5333969
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