Diversity and evolution of class 2 CRISPR-Cas systems.
Authors | |
Abstract | Class 2 CRISPR-Cas systems are characterized by effector modules that consist of a single multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the discovery of novel class 2 variants and used it to identify six new CRISPR-Cas subtypes. The diverse properties of these new systems provide potential for the development of versatile tools for genome editing and regulation. In this Analysis article, we present a comprehensive census of class 2 types and class 2 subtypes in complete and draft bacterial and archaeal genomes, outline evolutionary scenarios for the independent origin of different class 2 CRISPR-Cas systems from mobile genetic elements, and propose an amended classification and nomenclature of CRISPR-Cas. |
Year of Publication | 2017
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Journal | Nat Rev Microbiol
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Volume | 15
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Issue | 3
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Pages | 169-182
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Date Published | 2017 03
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ISSN | 1740-1534
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DOI | 10.1038/nrmicro.2016.184
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PubMed ID | 28111461
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