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Philos Trans R Soc Lond B Biol Sci DOI:10.1098/rstb.2016.0021

Microevolutionary traits and comparative population genomics of the emerging pathogenic fungus Cryptococcus gattii.

Publication TypeJournal Article
Year of Publication2016
AuthorsFarrer, RA, Voelz, K, Henk, DA, Johnston, SA, Fisher, MC, May, RC, Cuomo, CA
JournalPhilos Trans R Soc Lond B Biol Sci
Date Published2016 Dec 05

Emerging fungal pathogens cause an expanding burden of disease across the animal kingdom, including a rise in morbidity and mortality in humans. Yet, we currently have only a limited repertoire of available therapeutic interventions. A greater understanding of the mechanisms of fungal virulence and of the emergence of hypervirulence within species is therefore needed for new treatments and mitigation efforts. For example, over the past decade, an unusual lineage of Cryptococcus gattii, which was first detected on Vancouver Island, has spread to the Canadian mainland and the Pacific Northwest infecting otherwise healthy individuals. The molecular changes that led to the development of this hypervirulent cryptococcal lineage remain unclear. To explore this, we traced the history of similar microevolutionary events that can lead to changes in host range and pathogenicity. Here, we detail fine-resolution mapping of genetic differences between two highly related Cryptococcus gattii VGIIc isolates that differ in their virulence traits (phagocytosis, vomocytosis, macrophage death, mitochondrial tubularization and intracellular proliferation). We identified a small number of single site variants within coding regions that potentially contribute to variations in virulence. We then extended our methods across multiple lineages of C. gattii to study how selection is acting on key virulence genes within different lineages.This article is part of the themed issue 'Tackling emerging fungal threats to animal health, food security and ecosystem resilience'.


Alternate JournalPhilos. Trans. R. Soc. Lond., B, Biol. Sci.
PubMed ID28080992
PubMed Central IDPMC5095545
Grant List614562 / / European Research Council / International
U19 AI110818 / AI / NIAID NIH HHS / United States