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Curr Opin Biotechnol DOI:10.1016/j.copbio.2017.06.001

Disruptive non-disruptive applications of CRISPR/Cas9.

Publication TypeJournal Article
Year of Publication2017
AuthorsSchmid-Burgk, JL
JournalCurr Opin Biotechnol
Volume48
Pages203-209
Date Published2017 Dec
ISSN1879-0429
Abstract

The bacterial type II Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR Associated (Cas) systems, and in particular Streptococcus pyogenes CRISPR-Cas9, have been broadly applied to edit the genome of bacterial and eukaryotic cells. Cas9, which is an RNA-guided programmable nuclease, is a powerful tool for disrupting protein-coding genes. Cas9 cleaves target sites to generate a double-strand break (DSB) that is repaired via an error-prone repair process, leading to insertion/deletion mutations and gene knockouts. However, Cas9 can also be used to modulate genome function without gene disruption, enabling base editing, transcriptional and epigenetic reprogramming, genome imaging, cellular barcoding, genetic recording, and genetic computation.

DOI10.1016/j.copbio.2017.06.001
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/28633080?dopt=Abstract

Alternate JournalCurr. Opin. Biotechnol.
PubMed ID28633080