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Nat Commun DOI:10.1038/s41467-017-00965-y

Scalable whole-exome sequencing of cell-free DNA reveals high concordance with metastatic tumors.

Publication TypeJournal Article
Year of Publication2017
AuthorsAdalsteinsson, VA, Ha, G, Freeman, SS, Choudhury, AD, Stover, DG, Parsons, HA, Gydush, G, Reed, SC, Rotem, D, Rhoades, J, Loginov, D, Livitz, D, Rosebrock, D, Leshchiner, I, Kim, J, Stewart, C, Rosenberg, M, Francis, JM, Zhang, C-Z, Cohen, O, Oh, C, Ding, H, Polak, P, Lloyd, M, Mahmud, S, Helvie, K, Merrill, MS, Santiago, RA, O'Connor, EP, Jeong, SH, Leeson, R, Barry, RM, Kramkowski, JF, Zhang, Z, Polacek, L, Lohr, JG, Schleicher, M, Lipscomb, E, Saltzman, A, Oliver, NM, Marini, L, Waks, AG, Harshman, LC, Tolaney, SM, Van Allen, EM, Winer, EP, Lin, NU, Nakabayashi, M, Taplin, M-E, Johannessen, CM, Garraway, LA, Golub, TR, Boehm, JS, Wagle, N, Getz, G, J Love, C, Meyerson, M
JournalNat Commun
Volume8
Issue1
Pages1324
Date Published2017 Nov 06
ISSN2041-1723
Abstract

Whole-exome sequencing of cell-free DNA (cfDNA) could enable comprehensive profiling of tumors from blood but the genome-wide concordance between cfDNA and tumor biopsies is uncertain. Here we report ichorCNA, software that quantifies tumor content in cfDNA from 0.1× coverage whole-genome sequencing data without prior knowledge of tumor mutations. We apply ichorCNA to 1439 blood samples from 520 patients with metastatic prostate or breast cancers. In the earliest tested sample for each patient, 34% of patients have ≥10% tumor-derived cfDNA, sufficient for standard coverage whole-exome sequencing. Using whole-exome sequencing, we validate the concordance of clonal somatic mutations (88%), copy number alterations (80%), mutational signatures, and neoantigens between cfDNA and matched tumor biopsies from 41 patients with ≥10% cfDNA tumor content. In summary, we provide methods to identify patients eligible for comprehensive cfDNA profiling, revealing its applicability to many patients, and demonstrate high concordance of cfDNA and metastatic tumor whole-exome sequencing.

DOI10.1038/s41467-017-00965-y
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/29109393?dopt=Abstract

Alternate JournalNat Commun
PubMed ID29109393
PubMed Central IDPMC5673918
Grant ListP30 CA014051 / CA / NCI NIH HHS / United States