Small-molecule reagents for cellular pull-down experiments.
Bioconjug Chem
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Abstract | Affinity purification of interacting proteins from cellular extracts is a powerful technique for identifying the cellular targets of small molecules. Affinity matrix-based small-molecule reagents are usually prepared by conjugating small molecules of interest to a solid support such as agarose. This protocol describes an efficient and robust method to immobilize small molecules containing a primary alcohol, a common functional group in small molecules, especially in small molecules prepared using diversity-oriented synthesis. This method comprises one element of a systematic approach to the target identification problem in chemical biology. |
Year of Publication | 2008
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Journal | Bioconjug Chem
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Volume | 19
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Issue | 3
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Pages | 585-7
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Date Published | 2008 Mar
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ISSN | 1043-1802
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DOI | 10.1021/bc700297j
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PubMed ID | 18193824
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Grant list | 2P01 CA-78048 / CA / NCI NIH HHS / United States
Howard Hughes Medical Institute / United States
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