Small-molecule reagents for cellular pull-down experiments.

Bioconjug Chem
Authors
Keywords
Abstract

Affinity purification of interacting proteins from cellular extracts is a powerful technique for identifying the cellular targets of small molecules. Affinity matrix-based small-molecule reagents are usually prepared by conjugating small molecules of interest to a solid support such as agarose. This protocol describes an efficient and robust method to immobilize small molecules containing a primary alcohol, a common functional group in small molecules, especially in small molecules prepared using diversity-oriented synthesis. This method comprises one element of a systematic approach to the target identification problem in chemical biology.

Year of Publication
2008
Journal
Bioconjug Chem
Volume
19
Issue
3
Pages
585-7
Date Published
2008 Mar
ISSN
1043-1802
DOI
10.1021/bc700297j
PubMed ID
18193824
Links
Grant list
2P01 CA-78048 / CA / NCI NIH HHS / United States
Howard Hughes Medical Institute / United States