Immobilization of actively thromboresistant assemblies on sterile blood-contacting surfaces.
Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.
|Year of Publication||
Adv Healthc Mater
|PubMed Central ID||
R01 HL056819 / HL / NHLBI NIH HHS / United States
R01 HL083867 / HL / NHLBI NIH HHS / United States
R01 HL106018 / HL / NHLBI NIH HHS / United States