Multidimensional chemical control of CRISPR-Cas9.

Nat Chem Biol
Authors
Abstract

Cas9-based technologies have transformed genome engineering and the interrogation of genomic functions, but methods to control such technologies across numerous dimensions-including dose, time, specificity, and mutually exclusive modulation of multiple genes-are still lacking. We conferred such multidimensional controls to diverse Cas9 systems by leveraging small-molecule-regulated protein degron domains. Application of our strategy to both Cas9-mediated genome editing and transcriptional activities opens new avenues for systematic genome interrogation.

Year of Publication
2017
Journal
Nat Chem Biol
Volume
13
Issue
1
Pages
9-11
Date Published
2017 Jan
ISSN
1552-4469
DOI
10.1038/nchembio.2224
PubMed ID
27820801
Links