Multidimensional chemical control of CRISPR-Cas9.
Nat Chem Biol
Authors | |
Abstract | Cas9-based technologies have transformed genome engineering and the interrogation of genomic functions, but methods to control such technologies across numerous dimensions-including dose, time, specificity, and mutually exclusive modulation of multiple genes-are still lacking. We conferred such multidimensional controls to diverse Cas9 systems by leveraging small-molecule-regulated protein degron domains. Application of our strategy to both Cas9-mediated genome editing and transcriptional activities opens new avenues for systematic genome interrogation. |
Year of Publication | 2017
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Journal | Nat Chem Biol
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Volume | 13
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Issue | 1
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Pages | 9-11
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Date Published | 2017 Jan
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ISSN | 1552-4469
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DOI | 10.1038/nchembio.2224
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PubMed ID | 27820801
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