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Nat Commun DOI:10.1038/ncomms7121

The adhesion G protein-coupled receptor GPR56 is a cell-autonomous regulator of oligodendrocyte development.

Publication TypeJournal Article
Year of Publication2015
AuthorsGiera, S, Deng, Y, Luo, R, Ackerman, SD, Mogha, A, Monk, KR, Ying, Y, Jeong, S-J, Makinodan, M, Bialas, AR, Chang, BS, Stevens, B, Corfas, G, Piao, X
JournalNat Commun
Date Published2015 Jan 21
KeywordsAnimals, Axons, Brain, Cell Lineage, Cell Proliferation, Cell Survival, Central Nervous System, Corpus Callosum, Disease Models, Animal, Female, Gene Expression Regulation, Humans, Magnetic Resonance Imaging, Male, Malformations of Cortical Development, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Microscopy, Electron, Transmission, Mutation, Myelin Sheath, Oligodendroglia, Optic Nerve, Receptors, G-Protein-Coupled, rho GTP-Binding Proteins, Signal Transduction, Tamoxifen

Mutations in GPR56, a member of the adhesion G protein-coupled receptor family, cause a human brain malformation called bilateral frontoparietal polymicrogyria (BFPP). Magnetic resonance imaging (MRI) of BFPP brains reveals myelination defects in addition to brain malformation. However, the cellular role of GPR56 in oligodendrocyte development remains unknown. Here, we demonstrate that loss of Gpr56 leads to hypomyelination of the central nervous system in mice. GPR56 levels are abundant throughout early stages of oligodendrocyte development, but are downregulated in myelinating oligodendrocytes. Gpr56-knockout mice manifest with decreased oligodendrocyte precursor cell (OPC) proliferation and diminished levels of active RhoA, leading to fewer mature oligodendrocytes and a reduced number of myelinated axons in the corpus callosum and optic nerves. Conditional ablation of Gpr56 in OPCs leads to a reduced number of mature oligodendrocytes as seen in constitutive knockout of Gpr56. Together, our data define GPR56 as a cell-autonomous regulator of oligodendrocyte development.


Alternate JournalNat Commun
PubMed ID25607655
PubMed Central IDPMC4302951
Grant ListR01 NS094164 / NS / NINDS NIH HHS / United States
R01 NS035884 / NS / NINDS NIH HHS / United States
P30 HD018655 / HD / NICHD NIH HHS / United States
R01 NS057536 / NS / NINDS NIH HHS / United States
F31 NS087801 / NS / NINDS NIH HHS / United States
R01 NS079445 / NS / NINDS NIH HHS / United States